HUMAN HYBRIDOMAS

Hybridoma antibody surface display

Combining cell surface display with the convenience of hybridomas for human and mouse monoclonal antibody discovery.

Hybridoma methods have been a mainstay of antibody cloning for over 40 years, but they are labor intensive. OCMS fusion partner cell lines accelerate hybridoma workflows by creating cells that surface-display their antibodies, for high-throughput screens with fluorescent antigens. OCMS human hybridomas can be preserved for repeat screening and NGS.

Advantages

On-Cell mAb Screening

• Each cell captures its own antibody
• Fluorescent antigen binding identifies cells of interest
• Simplifies multiplex screening
• After on-cell selection, hybridomas resume antibody secretion to enter conventional discovery workflows

Experimental flexibility

• Antibodies can be tested On-Cell or as conventional secreted mAbs
• Find ultra-rare clones and prevent hybridoma loss
• Can be used with any mouse or human B cell
• Preserve and re-screen libraries

working with ocms bio

• OCMS has a flexible sales/licensing model for hybridoma users
• Collaborate on high value discovery programs
• Royalty-free fee-for-service with customer-provided B cells